Oligodendrocyte differentiation from adult multipotent stem cells is modulated by glutamate

We used multipotent stem cells (MSCs) derived from the young rat subventricular zone (SVZ) to study the effects of glutamate in oligodendrocyte maturation. Glutamate stimulated oligodendrocyte differentiation from SVZ-derived MSCs through the activation of specific N-methyl-D-aspartate (NMDA) receptor subunits. The effect of glutamate and NMDA on oligodendrocyte differentiation was evident in both the number of newly generated oligodendrocytes and their morphology. In addition, the levels of NMDAR1 and NMDAR2A protein increased during differentiation, whereas NMDAR2B and NMDAR3 protein levels decreased, suggesting differential expression of NMDA receptor subunits during maturation. Microfluorimetry showed that the activation of NMDA receptors during oligodendrocyte differentiation elevated cytosolic calcium levels and promoted myelination in cocultures with neurons. Moreover, we observed that stimulation of MSCs by NMDA receptors induced the generation of reactive oxygen species (ROS), which were negatively modulated by the NADPH inhibitor apocynin, and that the levels of ROS correlated with the degree of differentiation. Taken together, these findings suggest that ROS generated by NADPH oxidase by the activation of NMDA receptors promotes the maturation of oligodendrocytes and favors myelination

Edad de menarquía e indicadores de adiposidad en universitarias del País Vasco

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Maila handiko futbolari gazteen ezaugarrien garapena eta eboluzioa

314 p.

HIV-1 Capture and Transmission by Dendritic Cells: The Role of Viral Glycolipids and the Cellular Receptor Siglec-1

Dendritic cells (DCs) are essential in order to combat invading viruses and trigger antiviral responses. Paradoxically, in the case of HIV-1, DCs might contribute to viral pathogenesis through trans-infection, a mechanism that promotes viral capture and transmission to target cells, especially after DC maturation. In this review, we highlight recent evidence identifying sialyllactose-containing gangliosides in the viral membrane and the cellular lectin Siglec-1 as critical determinants for HIV-1 capture and storage by mature DCs and for DC-mediated trans-infection of T cells. In contrast, DC-SIGN, long considered to be the main receptor for DC capture of HIV-1, plays a minor role in mature DC-mediated HIV-1 capture and trans-infection.

Irakaslearen esku hartzea psikomotrizitate saioan

[eus] Bernard Aucouturier pedagogoak sortutako praktika psikomotorrak, bide motrizetik abiatuta, haurraren heltze psikologikoa helburu duen metodoa da. Haurra bere garapenean ulertu eta lagundu ahal izateko psikomotrizitatearen beharraz kontziente izanik eta Aucouturier metodologia abiapuntutzat hartuta, lan honetan, hezitzaile bakoitzari bere praktika hobetzeko eta horren inguruan hausnartzeko erreferentzia markoa eskainiko zaio. Horretarako, bi urteko gela bateko irakaslearen esku hartzearen analisiaren bidez, jardunaren egokitasuna ebaluatzearekin batera, esku hartzea hobetzeko proposamena eskainiko da. Izan ere, hezitzailearen oinarrizko funtzioa praktika psikomotorrean modu ez zuzentzailean esku hartzeko egoerak sortzea da, horretarako formakuntza pertsonal eta profesional sendo eta zorrotza ezinbestekoa izanik.